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Product Info Summary
| SKU: | A11916 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-GZF1 Antibody
SKU/Catalog Number
A11916
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GZF1 Antibody (Catalog # A11916). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
GZF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GZF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11916)
Host
Rabbit
Contents
GZF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
GZF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GZF1. The immunogen is located within amino acids 600 - 650 of GZF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist.
Reactive Species
A11916 is reactive to GZF1 in Human
Applications
A11916 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
80.492kDa
Background of ZNF336
The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GZF1 antibody can be used for detection of GZF1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GZF1 in human heart tissue lysate with GZF1 antibody at 1 μg/mL.
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Immunohistochemistry of GZF1 in human kidney tissue with GZF1 antibody at 2.5 μg/mL.
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Immunofluorescence of GZF1 in human kidney tissue with GZF1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For GZF1 (Source: Uniprot.org, NCBI)
Gene Name
GZF1
Full Name
GDNF-inducible zinc finger protein 1
Weight
80.492kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
GDNF-inducible zinc finger protein 1; ZBTB23; zinc finger and BTB domain-containing protein 23 GZF1 JLSM, ZBTB23, ZNF336 GDNF inducible zinc finger protein 1 GDNF-inducible zinc finger protein 1|zinc finger and BTB domain-containing protein 23|zinc finger protein 336
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GZF1, check out the GZF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GZF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GZF1 Antibody (A11916)
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No publications found for A11916
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